Biopolymers and cell. 1985. Volume 1. № 3. 147 - 153
Yu. I. Gorlov, V. S. Kirillova, L. G. Zharova, L. I. Likhacheva, V. A. Kordyum
PLASMID DNA TRANSFORMATION OF YEAST CELLS TREATED WITH CHELATING AGENTS. THE ROLE OF LIPID PEROXIDATION IN INDUCING COMPETENCE IN YEAST
Summary
It is found that the pretreatment of yeast Saccharomyces cerevisiae (strains 746 and LL-20) cells with the chelating agents of transition metals (8-hydroxyquinoline, 1,10-phe-nanthroline) causes the appearance of competence for plasmid DNA transformation. The factors affecting the efficiency of yeast cell transformation are studied using 8-hyd¬roxyquinoline and double-replicon plasmid RB4. Transformant yield under application of such a method reached 4X103-2X104 transformations per 10 u.g of plasmid DNA. The chelating agents are shown to increase lipid peroxide formation in yeast. The addition of the lipid oxidation inhibitor (ionol, the synthetic antioxidant) during cell tre¬atment with 8-hydroxyquinoline decreases the transformant yield. The data obtained indi¬cate that the effect of chelators on the yeast competence for transformation may be due to their influence on the membrane lipid peroxidation.