Biopolymers and cell. 1985. Volume 1. № 5. 259 - 265

V. G. Bezlepkin, Yu. Yu. Malinovsky, A. I. Gaziev

RADIATION-INDUCED UNSCHEDULED SYNTHESIS OF DNA AND DNA POLYMERASE ACTIVITY AT THE NUCLEAR MATRIX

Summary

    At the nuclear matrix of UV- and Y-irradiated cells from Zajdela hepatoma the higher level of hydroxyurea-resistant DNA synthesis is observed in the initial (1.5-5 min) period of postradiation incubation in comparison with total nuclear DNA. But 1-|5-D arabinofuranosylcytosine-resistant radiation-induced DNA synthesis is the same both in the nuclear matrix and in the intact nuclei of these cells. Both DNA polymerase a and DNA polymerase |3 are shown to participate in DNA synthesis at the nuclear matrix. DNA polymerase fS is more strongly associated with the nuclear matrix of normal liver cells in comparison with nuclei of the regenerating liver. The poly-(ADP-ribose)synthetase activity is shown to be associated with the nuclear matrix. Under the activity inhibition the hydroxyurea-resistant DNA synthesis is, mainly, induced in the nuclear matrix.