Biopolymers and cell. 1986. Volume 2. № 1. 50 - 52
N. I. Sjakste, T. G. Sjakste
DETACHMENT OF DNA FROM THE NUCLEAR MATRIX IN GROWTH-ARRESTED TRANSFORMED CHINESE HAMSTER FIBROBLASTS
Summary
SV-40 transformed Chinese hamster fibroblasts were growth-arrested by incubation in the medium with lowered serum content. DNA-protein interactions in the cells were studied by the nucleoprotein-celite-chromatography method. Detachment of the DNA from the nuclear matrix was revealed in quiescent cells. After the cells were restimula-ted to proliferate the reassociation of DNA and nuclear matrix was observed. The weakeŽning of the bonds of a part of DNA with nuclear matrix proteins was also observed in quiescent fibroblasts.