Biopolymers and cell. 1986. Volume 2. № 5. 275 - 278
E. B. Paton, A. N. Zhivolup
INSERTION OF A DNA FRAGMENT BETWEEN TWO STRONG SIMILARLY ORIENTED PROMOTERS OF RECOMBINANT FILAMENTOUS PHAGE mp8tJrpoB INCREASES ITS STABILITY AND MAKES POSSIBLE AN OPPOSITE ORIENTATION OF THE CLONED E. coli rpoB GENE
Summary
A fragment of K phage DNA containing terminator of transcription (to) and terminal part of the oop-RNA gene was inserted into the polylinker area of M13 mp8 filamentous phage. The obtained phage was used to clone a Bglll fragment of pJC703 cosmid, containing E. coli genes rpIJ, rpIL and rpoB together with promoters Pj and Pa. Stability of the obtained mp8t /rpoB recombinant phages increased up to 93 % and an orientation of the cloned Bgi//-fragment opposite to the previously observed one became possible.