Biopolymers and cell. 1986. Volume 2. 6. 317 - 322

 

Yu. F. Drygin, K. L. Zuklys

 

SUPERCOILED DNA AS A SUBSTRATE FOR DETECTION OF ENDONUCLEOLYTIC ACTIVITIES IN COMMERCIAL AND LABORATORY BIOCHEMICAL PREPARATIONS

 

Summary

 

    Supercoiled DNA is known to be a sensitive substrate for detecting the endonucleolytic activity. Supercoiled pBR322 and other Co/£7-related plasmids are shown to be highly useful both for detecting nonspecific endonucleolytic admixtures in many biochemical preparations and for comparative estimation of certain DNAses and RNAses activity.

 

Summary in Russian