Biopolymers and cell. 1987. Volume 3. № 1. 35 - 40

I. E. Kostetsky, V. A. Kordyum

LIPOSOME-MEDIATED DELIVERY OF DNA TO SACCHAROMYCES CEREVISIAE PROTOPLASTS

Summary

    To determine the peculiarities of plasmid DNA delivery by liposomes to yeast protoplasts (Saccharomyces cerevisiae) plasmid RB4 was encapsulated into large monolamellar liposomes from lecithin and cholesterin (mol, ratio 5:1). Fusion of liposomes with protoplasts was carried out. The uptake of plasmid DNA incorporated into liposomes by competent yeast cells was shown to exceed 8-12 fold than of free DNA. Liposomes without incorporated DNA were found to promote transformation of S. cerevisiae protoplasts 5-8 fold, if DNA concentration in a sample was 20-40 (xg/ml. The effect was absent at higher DNA concentrations in a sample (150-200 fig/ml).