Biopolymers and cell. 1987. Volume 3. № 3. 137 - 139
I. D. Golovatsky, B. A. Savchik
CONCANAVALIN-A APPLICATION FOR THE ISOLATION OF GLYCOPROTEINS AND THEIR ELECTROPHORETIC SEPARATION PARALLEL WITH OTHER PROTEINS
A combination of the methods for precipitation of glycoproteins by concanavalin-A. for their release by monosaccharides (glucose, mannose) and for subsequent separation in the polyacrylamide gel has been used for simultaneous -and parallel determination of certain glycoproteins and other .tissue proteins. A good reproducibility of the results is established. 10-20 fractions of glycoproteins being, mainly, in bands corresponding to other proteins are shown ito be present in different rat and bovine tissues.