Biopolymers and cell. 1987. Volume 3. № 4. 201 - 207

A. S. Fridman, D. Yu. Lando

EFFECT OF SELECTIVE LIGAND BINDING ON THE MOLECULAR DNA MELTING

Summary

    Short non-interacting ligands with a selective binding have been studied for their influence on the DNA helix-coil transition. It is shown that a direct binding of such ligands gives the same characteristics of the transition as the influence of dissolved substances on the heat DNA denaturation. The difference in characteristics is observed only at high temperature shifts (more than 30 °C) induced by ligands, where the dependence of the melting point on the GC-content of the complex is nonlinear. It is established that ligands induce the same temperature shifts but different behaviour of the dependence of the melting range width on the concentration.