Biopolymers and cell. 1987. Volume 3. № 6. 294 - 301
V. I. Bruskov, V. P. Kutyshenko
SPECIFICITY OF INTERACTION OF QLYCINE AND ITS METHYL DERIVATIVES WITH NUCLEIC ACID BASE FROM NUCLEAR MAGNETIC RESONANCE DATA
Summary
Interaction of glycine and its O- and N-methyl derivatives with nucleic acid bases have been investigated by the NMR method. O-methylglycine selectively interacts with cytosine and guanine in dirnethylsulphoxide (DMSO) solution. Cytosine forms a complex where NH+3-group of O-methylglycine specifically interacts with O2 and N3 atoms with simultaneous formation of hydrogen bonding between carbonyl group and NH2-group of cytosine. For guanine the complex formation is, probably, due to interaction of NH+ group with O6, N7 atoms and hydrogen bonding of carbonyl group with Nl-H-group. The methylglycine derivatives form complexes with cytosine and guanine and compete for hydrogen bonds formation in guanine-cytosine pairs in DMSO: H2O (3:1) solutions. But O-methylglycine forms a complex preferably with cytosine, whereas N-methylglycine — with guanine. The data obtained indicate the interaction of glycine with cytidine, CMP and GMP in water.