Biopolymers and cell. 1987. Volume 3. № 6. 307 - 312

E. B. Paton, I. V. Kroupskaya, A. N. Zhyvoloup

PARTICULARITIES OF CLONING OF ESCHERICHIA COLI rpoBC-OPERON FRAGMENTS IN THE pUC PLASMIDS

Summary

    Recombinant plasmids with inserted BglII E. coll rpoBC-op&ron fragment, including the whole rplL, rplJ and rpoB-genes and parts of rplA and rpoC-genes are constructed, plasmid pUC19 and cosmid pJC703 being the origin of them. The recombinant plasmid pUCIS with an inserted strong promoter of the rpoBC-operon — Pj as well as recombinant plasmids pUCI8 and pUC19 carrying the 2870 bp central fragment of the rpoB-gene are also constructed. Orientations of the cloned fragments are determined for all the recombinant plasmids. It is supposed that strong Pj-promoter including part of the BglII pJC703 fragment determines its orientation when it is inserted into the pUC plasmid.