Biopolymers and cell. 1987. Volume 3. № 6. 320 - 322

Huynh Xuan Tfiao, Trinh Mank Dung, Ngo Ke Suong

A SIMPLE METHOD FOR ISOLATION OF HAPLOID PROTOPLASTS FROM FORMS OF RICE MULTISHOOTS

Summary

    One-month old sterile plantlets grown on the 0.3 M sucrose medium were used for protoplast isolation. The young part of leaves were cut onto small pices (1 mm) and left for over night incubation in the 2 % v/v driselase solution containing glucose 0.4 M. Isolated protoplasts were purified by two-phase centrifugation (the upper phase contains solution of salts, the lower one — 0.4 M of sucrose and other nutritional constituents). The purified protoplasts were suspended between two phases.