Biopolymers and cell. 1988. Volume 4. № 2. 91 - 100

I. B. Alieva, I. A. Vorobjev

NOCODAZOLE INACTIVATES MICROTUBULE ORGANIZING CENTRES AND INDUCES THE FORMATION OF LOCAL ZONES OF MICROTUBULE POLYMERIZATION IN K-MITOSIS

Summary

    Under the action of nocodasole at a concentration of 0.02 ng/ml the mitotic spindle is preserved in PE cells. Nocodazole at a concentration of 0.2 ng/ml results in the appearance of microtubule (MT) convergence centres lacking the centrioles and/or chromosomes. Bundles of MT bypass kinetochores, kinetochores become 1.5-3-times as long. At a nocodazole concentration of 0.02-0.2 [ig/ml the daughter centriole becomes 1.5-2-fold as long. Under the action of nocodazole at a concentration of 0.6 [ig/ml all chromosomes are packed in a slot-like structure. Kinetochores of several chromosomes surrounding one «slot» may be situated on their inner surfaces. Then short disorderly MT are observed in such a «slot». The centrioles are not surrounded by MT. In each cell one diplosome (rarely both) disintegrate. It is supposed that the appearance of three- and tetrapolar mitoses in tissue culture cells is due to the disintegration of one or both diplosomes, respectively.