Biopolymers and cell. 1988. Volume 4. № 5. 227 - 232

I. V. Razlutsky, L. S. Shlyakhtenko, Yu. L. Lyubchenko

THE INFLUENCE OF BASE-PAIR CHANGES ON DNA DENATURATION PROFILES

Summary

    Melting profiles of DNA fragments of ~1150 b. p. long containing deletions of one, five and six nucleotides have been obtained. It was shown that all these deletions chan¬ged the position only of one peak on the DNAs melting profiles. The value of the shift was 0.28rb0.3 °C in the case of deletion of one GC and four AT base pairs and it was 0.14+0.03 °C in the case of deletion of one GC-base pair. Denaturing gradient gel electrophoresis was also used for investigation of these DNA fragments melting. The deletion of one GC-base pair was demonstrated to cause a substational shift of the DNA denaluration profile