Biopolymers and cell. 1988. Volume 4. № 5. 250 - 254

V. V. Vlassov, Yu. D. Krendelev, M. N. Ovander, A. S. Ryte, V. E. Repin, F. P. Svinarclwk

THE EFFICIENT METHOD FOR DNA ENCAPSULATION INTO RECONSTITUTED SENDAI VIRUS ENVELOPES

Summary

    The freezing-thawing method is used for encapsulation up to 17000 base pairs DNA into reconstituted Sendai virus envelopes (RSVE). It permits encapsulating up to 30 % of DNA added to the solution. The interaction RSVE loaded with plasmid DNA and Krebs-2 ascites tumours cells results in up to 30 % penetration of the encapsulated material into the cells.