Biopolymers and cell. 1988. Volume 4. № 6. 283 - 289

M. P. Perelroyzen, A. V. Vologodskii

THEORETICAL ANALYSIS OF ADDRESSED MODIFICATION OF DNA

Summary

    Specific chemical addressed modification of DNA involves treatment of single-stranded DNA by oligonucleotides complementary to certain target sequences in this DNA and bearing groupings reactive towards the DNA bases. The binding with the partially comple¬mentary targets can cause non-specific modification. The selectivity of the addressed modification is the subject of the present investigation. Modification of a fragment is ana¬lyzed which is flanked by two target sequences complementary to a given oligonucleotide address and contains no more such exact targets over all its length. Conditions of maximum ratio between specific and non-specific modification are calculated. The probability of the process in which up to the given time both of the target termini will be specifically modified without any non-specific modification of the internal part of a fragment is determined.