Biopolymers and cell. 1989. Volume 5. № 5. 70 - 75

I. S. Croisman, A. P. Potapov

STUDIES IN CORRELATION BETWEEN POLY(U) MISREADING AND EFFICIENCY OF POLY(dT) TRANSLATION IN THE CELL-FREE PROTEIN-SYNTHESIZING SYSTEMS OF E. coli

Summary

    The efficiency of poly(dT) translation and misreading of poly(U) has been studied in cell-free systems from wild-type E. coli and streptomycin-resistant mutants with altered ribosome protein S12. The data show that there is a positive correlation between poly(U) misreading and efficiency of poly(dT) translation. Conditions promoting misreading of poly(U): an increase in the concentration of neomycin, kanamycin, streptomycin antibiotics, of Mg2+ ions stimulate poly(dT) translation as well. A series of antibiotics activities in promotion of both poly(U) misreading and poly(dT) translation is the same: neo-, mycin> kanamycin > streptomycin. The more accurate ribosomes with altered protein SI2 are less efficient in translation of poly(dT). T he data obtained are in good agreement with the hypothesis on stereospecific stabilization of codon-anticodon complexes on the ribosome.