Biopolymers and cell. 1989. Volume 5. № 6. 87 - 92

S. B. Zolotukhin, E. Yu. Markelova, G. V. Panasenko, V. G. Naidenov, A. D. Shved, Yu. Yu. Sautin, Ya. V. Patsko

A FAST METHOD FOR CLONING OF SPECIFIC cDINA. CLONING OF HUMAN PROLACTIN cDNA

Summary

    A method allowing fast and effective cloning of particular cD'NA is described. Pairs of synthetic oligonucleotides-primers are used for polymerize chain reaction mediated selective amplification of prolactine cDNTA synthesized on human pituitary poly (A)-mRiNA. Sequence of one of the recombinant plasmid insert revealed a full-length pro-lactin cDNA, flanked by the synthetic primers.