Biopolymers and cell. 1990. Volume 6. № 2. 31 - 35
N. B. Dolzhanskaya, M. Yu. MandelMam, E. L. Pat kin, A. S. Kuznetsov, F. L. Vikhanskaya, R. L KruUlina, V. S. Gaitskhoki
A MODEL FOR GENETIC CORRECTION OF FAMILY HYPERCHOLESTEROLEMIA
Summary
The expression of human low-density lipoprotein receptor (rLDL) was studied in mouse fibroblasts (1-929 line) transformed by the pMSVL recombinant plasmid. This plasmid contains full-length rLDL cDNA and hybrid promoter composed of regulatory elements from early SV40 promoter and glucocorticoid-responsive enhancer of Molony sarcoma virus. The expression of rLDL c DNA in transformed cells was demonstrated using dot and blot RNA-cDNA hybridization as well as immunofluorescent analysis.