Biopolymers and cell. 1990. Volume 6. № 4. 51 - 59

N. Yu. Miryuta, L. I. Strokovskaya, I. N. Skuratovskaya

STRUCTURAL CHARACTERISTICS OF THE INTEGRATION REGIONS OF NPV DNA AND HOST CELL IN THE GALLERIA MELLONELLA GENOME

Summary

    The physical state of the viral genome in the cell and certain structural characteristics of binding sites of virus and cell sequences in Gallerla mellonella DNA and NPV DNA have been investigated. The possibility of covalent bond between genome DNAs of virus and cell is shown. The studies of kinetic and thermal fractions obtained from. G. mello¬nella larvae of the Prague stock for DNA with different weights have permitted locali¬zing NPV DNA regions containing homology regions, showing linkage between virus and cell DNA in the cell genome, investigating virus DNA regions adjacent to cell DNA. This DNA proved to contain homology regions and neighbouring virus DNA fragments. A model of integration of NPV genome with G. mellonella larvae genome has been suggested.