Biopolymers and cell. 1990. Volume 6. № 4. 59 - 65

T. V. Venkstern, D. M. Graijer, G. G. Karpova, I. A. Morozov

STUDIES IN THE INTERACTION OF THE tRNA^phe DERIVATIVE BEARING AN ARYL-AZIDE GROUP ON ITS G24 RESIDUE WITH ESCHERICHIA COLl RIBOSO-MES AND tRNA-(ADENINE-l-)METHYL TRANSFERASE FROM THERMUS THER-MOPHILUS

Summary

    Photoaffinity labelling of E. coli ribosomes and tRNA (adenine-1-)methyl transferase has been studied using E. coli tRNAphe derivative bearing an aryl-azide group on its Gj4 residue. Under UV-irradiation (A.>310 nm) this derivative is shown to be cross-linked to tRNA(adenine-l-)methyl transferase from T. thermophilus but being located at ribosomal A or P site no cross-linking of the tRNA derivative to the ribosomes is observed.