Biopolymers and cell. 1991. Volume 7. 2. 86 - 92

 

LIVSHITS L. A., KRAVCHENKO S. A., GRISHKO V. I., BUZHIEVSKAYA T. I., BARANOV V. S.

 

Disequilibrium Linkage Between Polymorphism of the Restriction Fragments Length in Human Chromosome 7 with Mutations in Mucoviscidosis Gene in the Ukrainian Population

 

Summary

 

    DNA analysis of deletion delta F508 was performed in 37 Ukrainian cystic fibrosis families with 1 : 25 rise of cystic fibrosis and 100 healthy donors used PCR technology. A delation of three base pairs was detected in 65 percent of chromosomes carrying cystic fibrosis mutation. The linkage analysis between three polymorphic markers (KM-19, CS.7, D7S8) and cystic fibrosis mutations was performed. The linkage disequilibrium was found between RFLP-gaplotypes of chromosome 7 (KM-19/PstI and CS7/Hin61) and CF-deletion delta Fsos. weaker but significant disequlibrium was found between KM~19/PstI and CS.7/ /Hin61 and unknown one. The linkage between these markes and CF-mutations for the Ukrainian population permit to perform accurate pre- and postnatal diagnosis cystic librosis in most families with affected persons and also allows carriers detection in population.