Biopolymers and cell. 1992; 8 (3): 23 -29

Detection of pUC19-Homologous Repeated Sequences Into Genome of Several Species of Higher Plants

PATSKOVSKY Yu. V., GAIDUK V. V., VESELOVSKY O. V., ZUBKO E. I.. PASTERNAK T. P., YURKEVITCH L. N., MASHTALER S. G., POTOPALSKY A. I.

Summary

    With Southern blot hybridization into a nuclear DNA of the several species of higher plants (Secale cereale, Zea mays, Trilicum qestivum, Solanum nigrum, S. tuberosum, Atropa belladonna, Licopersicon esculentum) the pUC19-homologous repeated DNA sequences are detected. In the genomic DNA of S. cereale and A. belladonna its are clustered. The difference to the pUC-repeats organization and to their copies number between several species and several lines (or generations — S. cereale) of each species is shown. The possibility of using of the pUC-homologous sequences as a marker for somatic hybridization is demonstrated. The possible correlation of the showed facts with plant genome instability is discussed.