Biopolymers and cell. 1992; 8 (3): 29 -32

NIKOLAEV A. I., TCHKONIA T. T., ERISTAVI-KAFIANI K. A., TARANTYL V. Z.

Summary

    With the aid of microinjection of plasmid pPrC-LTRl,5. harboring 1.5 DNA copies of Rous sarcoma virus (RSV) long terminal repeats (LTRs) in vector pBR322, transgenic silkworms (Bombyx mori L.) were obtained. The transgene was transmitted in three, obtained up to now, generation. Most of the exogenous DNA is not integrated into the genome and persist as an extrachromosomal element which was subject to rearrangements. Plasmids have been rescued that carry the part of injected DNA and also fragments of the silkworm cellular DNA. In one of the rescued plasmids a sequence was found that is a member of a family of evolutionally concerved repeated sequences.