Biopolymers and cell. 1992; 8 (3): 33 -36

 

Optimization of the System for Genetic-Embryological Studies of Inbred Mice Strains. 2. Comparative Characterization of Season Female Fertility of BALB/cLac, C57BL/6j and ICR Mice

 

MOROZOVA L. M., STOLINA M. R., SOLOMKO A. P.

 

Summary

 

    We present data on optimization of the biological system for in vitro fertilization without preliminary hormonal stimulation of inbred ova donors. An incubation, medium for in vitro fertilization was chosen, which is optimal for it least two different in many features inbred mice strains C57BL/6j and BALB/cLac. We demonstrated the male genotype is dominant during in vitro fertilization, while the female genotype appears to be dominant in the course of zygote development in culture until late preimplantation stages. Application of C57BL spermatozoa rises the effectivity of in vitro fertilization from 50 to 76 %. Maternal C57BL genotype ensures successful development of about 90 % of homo- and heterozygotes till blastocyst stage