Biopolymers and cell. 1992; 8 (3): 53 - 56

SHUL'GA N. I., POTAPOV A. P.

Summary

    The effect of different concentration of neomycin on poly(U) and poly(dT) translation has been studied in cell-free systems from S. cerevisiae and M. crassa. The data show that this translation is carried out by cytoplasmic ribosomes. Mg2+ iones stimulate poly(U) and poly(dT) translation in these systems and neomycin unlike the prokaryotic cell-free systems inhibits translation of both templates. It was proposed that ribosome decoding center was changing during evolution to higher stereospecificity to the codon-anticodon sugar-phosphate backbone structure.