Biopolymers and cell. 1992. Volume 8. № 6. 44 - 49

KRAVETZ O. A., POGREBNYAK N. Y., SHISHA Ye. N., LOPATO S. V., GLEBA Yu. Yu.

Transformation of Some Potato Cultivars and Analysis of Transgenic Plants

Summary

    Kanamycin resistant plants of Solanum tuberosum L. (cv. Lugov-skoy, Desiree) and Nicotiana tabacum SR-1 were obtained following the cocultivation of stem segments and leaf discs with Agrobacterium tumefaciens. A disarmed binary vector system containing the neomycin phosphotransferase (NPT II) gene and gene encoding mutant acetolactate synthase (ALS) from Arabidopsis thaliana was used. Transformation was confirmed by the ability of stem segments to initiate callus on me¬dium containing 100 mg/1 kanamycin and Southern blot analysis.