Biopolymers and cell. 1992. Volume 8. № 6. 50 - 54

BOULANGER ANDRES, A., IVANSKAYA Naila V.

Comparison of Solid-Phase A-Protein Immune Electron Microscopy, Direct Electron Microscopy and Dot-ELISA for Detection of Transmissible Gastroenteritis Virus in Feces

Summary

    The authors have shown the solid-phase A-protein immune electron microscopy permits, to detect the fecal coronavirus with the highest sensitivity (54.9 % of 171 fecal samples studied) comparing to direct electron microscopic examination (39.1 %). It is possible to identifity transmissible gastroenteritis virus and to differ it from other virus partic¬les. Dot-ELlSA method has shown to demonstrate viral antigen in 51.4 % of the 171 samples investigated. So the solid-phase immune electron microscopy and dot-ELLSA are convenient for the veterinary diagnostics.