Biopolymers and cell. 1993. Volume 9. № 5. 101 - 106

BOGDARINA I G., MYRCHOVA M. I., BURYANOV Ya. I.

Heat Inducible Inhibition of the Neomycin Phosphotransferase II Activity in Transgenic Tobacco Cells

Summary

    Double transformed tobacco plants were obtained by successive transformation using two nptll gene constructs: the sense gene under the control of the constitutive nos promoter and the antisense form under the control of the inducible Drosophila melanogaster hsp70 promoter. Virtually total inhibition of the neomycin phosphotransferase II activity was observed in these plants only in response to heat shock. The same effect was observed in experiments on transient expression of the nptll gene in protoplasts of single transformed plants carrying the antisense npill gene under the hsp70 promoter. Therefore, the hsp70 promoter can be used in anlisens'e gene construction to inducibiy repress gene expression in transgenic plants.