Biopolymers and cell. 1995; 11 (1): 61 - 65

LYSOGENY BY MS2 PHAGE. ANALYSIS OF A RECOMBINANT PLASMID CONTAINING MS2 RNA-LIKE SEQUENCE

T. Pererva, N. Mirjuta, A. Mirjuta, M. Woodmaska, E. Zherebtsova

pLS4 recombinant plasmid coding MS2-llke RNA synthesis in plasmid-containing cells has been studied using physical mapping and blot-hybridisation with P32-labelled pMS27 plasmid fragments including a DNA copy of MS2 phage RNA. The authors have determined the size of a fragment containing MS2-like sequence and its localisation inside the hybrid plasmid.