Biopolymers and cell. 1995; 11 (2): 39 - 45

STRUCTURAL AND FUNCTIONAL ANALYSIS OF METHYLOTROPHIC YEAST HANSENULA POLYMORPHA ALCOHOL OXYDASE GENE PROMOTER

O. P. Vitvitskaya, M. L. Zlochevskii, M. Yu. Beburov

The influence of deletions in the AOX promoter on the Escherichia coll lacZ and the H. polymorpha AOX gene expression has been studied. It was shown, that the region between —403 bp and ATQ codon is absolutely necessary for gene transcription and its regulation in the methylotrophic yeasts. The upstream activating sequences (UASS) of the AOX promoter have been determined. The differences of gene expression regu¬lation under the control of the AOX promoter in the yeast H. polymorpha and Saccha-romyces cerevislae have been found.