Biopolymers and cell. 1995; 11 (3-4): 35 - 41

PREPARATION OF THE FLUORESCE1N REAGENT FOR SOLID-PHASE OLIGONUCLEOTIDE 5'-LABELLING AND ITS USE FOR THE SYNTHESIS OF FLUORESCENTLY LABELLED PCR PRIMERS FOR HIV-1 DETECTION

I. Ya. Dubey, 0. D. Fedoryak, 0. M. Tkachak, D. M. Fedoryak

The preparative synthesis of fluorescein H-phosphonate reagent for solid phase 'labelling of oligonucteotides is described. Fluorescein-labelted thymidine and two PCR primers for HIV-1 detection were synthesized using this reagent.