Biopolymers and cell. 1995; 11 (5): 46 - 50

ACTINOMYCIN D-INDUCED OSCILLATIONS IN THE INTENSITY OF NUCLEIC ACIDS SYNTHESIS IN LEUKEMIA CELLS P-388

/. M. Danko, V. t. Prima

The effect of actinomycin D on the intensity of nucleic acids synthesis has been stu¬died on leukemia cells P-388. It was found, that not only changes in degree of inhi¬bition of RNA synthesis rate are related to the antibiotic concentration, but also kine¬tics of the process itself. Certain actinomycin D doses are found to cause cyclical vari¬ations in the DNA and RNA synthesis rate. But inhibition and activation periods alter¬nating during the DNA and RNA synthesis coincide in time only partially and inhibi¬tion phase for RNA is two times the corresponding phase for DNA. Changes detected seem to have direct relationship with transcription regulation mechanisms.