Biopolymers and cell. 1996; 12 (2): 47 - 55

Kanamycin resistance of tobacco plants influenced by the preparations of DNAs on seeds

V. A. Katsan, A. I. Potopalsky, M. E. Les'kiv

The direct influence of the exogenic pCAMVNEO DNA (eDNA) has been investigated on kanamycin resistance of the tobacco plants cultivar Krupnolystny 20 in the first and second generations ( Tl and T2J. The pCAMVNEO DNA had been effected on the seeds in the aqueous solutions being applied separately as well as in a mixture with eDNAs originated from pTi8628 and tlie salt-tolerant black niglishade (Solatium nigrum). Kanamycin-resistant plants were obtained in considerable amount in all variants of experiment. The progeny of the resistant in Tl plants proved to be kanamycin-sensitive in 7'2. The correlation between the kanamycin resistance and the content of'fundamental photosynthetic pigments was notrevealed among the most variants of the experiment. The possible kanamycin resistance mechanisms of tobacco in T{ are being discussed.