Biopolymers and cell. 1996; 12 (6): 63 - 68

Molecular-biology approaches to detection of Philadelphia chromosome in patients with leukemia

G. D. Telegeev, M. V. Dybkov, M. V. Bozhko, N. M. Tretiak, S. S. Maliuta

The data on Philadelphia chromosome detection in patients with CML and ALL are presented. Two ap¬proaches were applied: .5' and 3' bcr genomic probes were used; two steps PCR with specific primers was employed. The latter is more preferable for clinic use. Some issues of Ph' leukemias etiology are also dis¬cussed.