Biopolymers and cell. 1996; 12 (6): 69 - 73

Characterization of catalytic activity in vitro of the ribozyme targeted to tat-RNA of HIV-I

L. N. Buryanovsky, A. D. Shved

Essential properties of catalytic activity in vitro of the anti-tot RNA (HIV-I) hammerhead ribozyme have been studied. Spermidine-dependencies, discovered by us earlier, has supported. Optimal concentration of magnesium ion were established and was equal 5—10 mM. Temperature optimum of reaction was 30 °C. 20 % formamide has stimulating influence on efficiency of the cleavage at 15 "C. Time-dependence course of efficiency of reaction also were carried out.