Biopolymers and cell. 1996; 12 (6): 74 - 81

Interaction of cyanine dyes with nucleic acids. 2. Spectroscopic properties of methyleneoxy analogues of Thiazole Orange

Sergiy M. Yarmoluk, Vladyslava B. Kovalska, Tetyana V. Smirnova, Mykola P. Shandura, Yuriy P. Kovtun, Gennadiy Kh. Matsuka

A series of asymmetric cyanine dyes based on methylenoxy benzothiazole terminal hetero-cycle was synthesized. Absorption and fluorescent properties of these dyes and their comp¬lexes with nucleic acids were investigated. The best results were obtained with 2-[(3-methyl-2(3H)-benzothiazolyliden)-methyl]-5,6-dioxymethylene-3-methylbenzothiaz olium p-toluenesulfonate (Cyan 13). A possible model of binding monomethyne benz-thiazole cyanine dyes with double-stranded nucleic acids is proposed.