Biopolymers and cell. 1997; 13 (2): 121 - 126

Cloning of cDNA encoding C-terminal part of mammalian tyrosyl-tRNA synthetase using of PCR-amplified radioactive probe

O. V. Levancts, V. G. Naidenov, M. I. Woodmaska, G. H. Matsuka, A. I. Kornelyuk

Radioactive probe of high activity (3.5-10 cpm/fig) specific to mammalian tyrosyl-tRNA synthetase has been synthesized by polymerase chain reaction. Bovine liver cDNA-library has been screened and specific clone has been detected. Sequencing of cDNA insert shows that the done encodes C-terminal part of bovine tyrosyl-tRNA synthetase. C-terminal non-canalytic domain of mammalian tyrosyl-tRNA synthetase shares homology of amino acid sequence with a novel cytokine — endothelial-monocyte activating polypeptide 11.