Biopolymers and cell. 1998; 14 (2): 163 - 176

Synthesis and use of disulfide-based H-phosphonate reagent for 3'- and/or 5'-oligonucleotide labelling via inercaptoalkyl linker

I. Y. Dubey, D. M. Fedoryak

Synthesis of new disulfide-based H-phosphonate reagent for solid-phase oligonucleotide functionalization with mercaptopropyl group at one or two termini is described. Using this single reagent, 3'- and 3', 5'-disulfide-linked oligonucleotides were synthesized, disulfide bonds cleaved with dithiothreitol and generated thiol groups labelled with iodoacetamidofluorescein. Tis oligonucleotides containing one or two fluorescein residues at 3'- and 5'-ends were prepared in high yields.