Biopolymers and cell. 1999; 15 (5): 367 - 373

Synthesis and study of antisense oligonuclcolides modified with imidazophenazine nucleosides

V. L. Makitruk, A. S. Shalamay, I. Y. Dubey, D. M. Fedoryak

Oligonucleotides containing imidazophenazine nucleosides at 3'-, 5'-and internal positions, which are complementary to certain regions of ribosomal operon DNA and I6S rRNA of mycoplasmas and E. co/i, have been synthesized by H-phosphonate solid phase method. It has been shown that nucleosides of imidazol4,5-b)phenazine and its 2-methyl analog as efficient intercalating agents strongly affect the stability of complementary complexes increasing the antisense activity of modified otigonucleotide sequence.