Biopolymers and cell. 2000; 16 (4): 281 - 283

Study of different physical and chemical parameters of non-canonical complex of eukaryotic translation elongation factor 1A with deacylated tRNA

T. V. Budkevich, B. S. Negrutskii, I. N. Serdyuk, A. V. EFskaya

For the first time the KA of [eEF-lA-GDP] with free tRNA was determined by steady state fluorescence polarization. The obtained value — 20 nM is allowed to suppose a physiological significance of the complex investigated. For the first time the solution con¬formation of rabbit liver [eEF-lA-GDP] and its complex with tRNA has been studied by small-angle neutron scattering (SANS). The shape of the [eEF-lA-GDP] molecule in solution is demonstrated to be rather relaxed and to undergo rather substantial changes upon formation of the complex with tRNA. The complex has a more compact structure than free [eEF-lA • GDP].