Biopolymers and cell. 2000; 16 (6): 482 - 486

Mutation in bcr/abl hybrid gene as a possible factor of tumor progression during CML

TELEGEEV G. D., DUBROVSKA A. N.. DYBKOV M. V., MALIUTA S. S.

The bcr/abl hybrid gene is a hallmark of CML and of many cases of acute leukemias (ALL). The expression of this gene is the condition of the beginning or one of the stages of these diseases. In contrast to ALL, the bcr/abl fusion gene in CML has additional 3—10 exons (Dbl and PH domains). We analyzed this region seeking a cause of the blast crisis. The sequencing of RT/PCR product has revealed the point mutation at nucleotide 2109 of bcr/abl mRNA (the transition mutation from T to C) causing the amino acid change from Phe547 to Leu547. Using FITC-phalloidin we have shown the paramembrane actin location.