Biopolymers and cell. 2001; 17 (3): 237 - 241

Gene co-integration at simultaneous transformation of Arabidopsis thaliana plants by different vectors using the in planta method

V. V. Radchuk

As a result of in planta transformation of Arabidopsis thaliana plants by using Agrobacterium mixture, containing three different constructions, 68 transgenic lines have been obtained High fre¬quency of gene co-integration due to the transformation is reported. Twenty three (34 %) plants possessed two T-DNAs from different constructions, while six plants (ca. 9 %) were with all three T-DNAs. It has been concluded that the efficiency of gene transfer does not depend on the gene length but mainly on competence of the recipient plant cells.