Biopolymers and cell. 2001; 17 (3): 253 - 255

Formamide and glycerol in sequencing of GC-rich DNA

S. M. Kvasha, E. R. Zabarovsky, V. I. Kashuba, A. V. Rynditch

Formamide and glycerol are known to increase the sequencing efficiency. We compared the ability of different concentration of these co-solvents to improve the sequencing results of GC-rich template. Herein, we report that addition of 5 % formamide and 7.5—JO % glycerol is the most effective for sequencing GC-rich templates.