Biopolymers and cell. 2001; 17 (6): 546 - 550

The expression of human alpha-2b interferon in different strains of Escherichia coli

I. Yu. Slavchcnko

The expression of human alpha-2b-interferon (IFN) in various strains of E. coli carrying the same recombinant plasmid plF-16 has been investigated. The level of IFN synthesis in different E. coli straines is shown to vary from electrophoretically undetectable amount to 35 % of the total cell proteins under the identical conditions of producer cultivation. The difference in the levels of interferon synthesis is not related to genetic instability of the plasmids.