Biopolymers and cell. 2002; 18 (4): 356 - 358

Infrared absorption spectra of nucleic acids: the attenuated total reflection technique

A. V. Stepanyugin, O. I. Martynenko, S. P. Samijlenko, D. M. Hovorun

For the first time the attenuated total reflection (ATR) technique has been applied to obtain the JR absorption spectra of DNA and RNA. The procedure of removing NA films from a germanium prism has been worked through, thus a main obstacle in an appication of this technique to nucleic acids has been overcome.