Biopolymers and cell. 2002; 18 (6): 485 - 488

Restriction polymorphism of amplified fragments of the genus Nicotiana chloroplast DNA

S. I. Komarnytskyy, I. K. Komarnytskyy

Using the PCR amplification of short regions of the chloroplast DNA together with their subsequent restriction digestion, it is possible to follow the cytoplasm inheritance during the genus Nicotiana development. The data obtained confirmed the cultivated tobacco cytoplasm to originate from the Rustica subgenus ancestors. Two Australian species, N. debneyi and N. fragrans, have nothing in common with, the cytoplasm of other species of Suaveolentes section of the genus.