Biopolymers and cell. Volume 20. № 1-2. 17 - 23.
P. V. Futernyk, A. P. Pogribna, Z. M. Petrushenko, B. S. Negrutski, G. V. El'skaya
Investigation of the complexes of elongation factor 1A with tRNASer and seryl-tRNA synthetase
To investigate the mechanism of tRNA channeling the formation of non-canonical complexes of elongation factor 1A1 with deacylated tRNASer and seryl-tRNA synthetase was studied using band shift assay. The stability of these complexes was estimated. The formation of non-canonical complex of tRNA with the tissue-specific isoform of elongation factor 1A2 was examined and found to be more stable than the analogous complex with eEF1A1. Our results are in accordance with the proposed earlier mechanism of tRNA channeling during the elongation cycle of protein biosynthesis.