Biopolymers and cell. Volume 20. 1-2. 34 - 40.


V. V. Dmitrenko, K. O. Shostak, O. I. Boyko, O.V. Khomenko, V. D. Rozumenko, T.A. Malisheva, M. I. Shamayev, Yu. P. Zozulya, V. M. Kavsan


Changes in the expression of mitochondrial genes in human glioblastoma




80 cDNA clones differentially hybridized with total cDNA probes of glioblastoma and normal human brain were revealed by differential hybridization of human fetal brain cDNA library. Hybridization of the DNA of selected clones with the same cDNA probes verified the difference for 38 clones. The levels of six nucleotide sequences from 6 clones containing Alu-repeats were increased in glioblastoma. 20 clones contained cDNAs corresponding to mitochondrial genes for ATP synthase subunit 6 (ATP6), cytochrome C oxidase subunit II (COXII), cytochrome C oxidase subunit III (COXIII), NADH dehydrogenase subunit 1 (ND1), NADH dehydrogenase subunit 4 (ND4) and mitochondrial 12S rRNA. The expression of all these mitochondrial genes was decreased in glioblastomas as compared to tumor-adjacent normal brain.