Biopolymers and cell. 2005; 21 (1): 35 - 41

Intertribal somatic hybrids of di- and tetragenome origin: production and usage

O. O. Ovcharenko, I. K. Komarnytskyy, M. M. Cherep, V. A. Rudas, M. V. Kuchuk

Intertribal hybrids in the combinations of Orychophragmus violaceus (L.) O. E. Schulz.+Arabidopsis thaliana L. and O. violaceus (L.) O. E. Schulz.+hybrid (Brassica juncea (L.) Czern. & Coss+A. thaliana L.) were obtained as a result of somatic hybridization. Hybrid nature of the plants obtained was confirmed by the PCR-RFLP and analysis of isozymes. A. thaliana L. plants were transformed with a vector carrying heterologous transposable element Spm/dSpm and genes nptII, bar, gus, which allowed to examine the behavior of transgenic traits in digenomic and tetragenomic hybrids. The hybrid plants were similar to one of the parental species (O. violaceus+A. thaliana) or had intermediate morphology (O. violaceus+hybrid (B. juncea+A. thaliana)). All the plants obtained were resistant to kanamycin and phosphinothricin due to the transgenes transferred from A. thaliana. The activity of b-glucuronidase was detected in hybrid O. violaceus+A.thaliana while it was absent in the plants O. violaceus+hybrid (B. juncea+A. thaliana). PCR analysis revealed maintenance of all transgenes in the hybrids.

Key words: intertribal somatic hybridization, Orychophragmus violaceus, Brassica juncea, Arabidopsis thaliana, heterologous system of transposable element Spm/dSpm.