Biopolymers and cell. 2005; 21 (4): 346 - 350

Multibiosensor on the basis of yeast cells for detection of glucose and sucrose in aqueous solutions

A. L. Kukla, M. I. Kanyuk, M. F. Starodub, S. S. Nagornaya

A multichannel biosensor based on capacitive structures of electrolyte-insulator-semiconductor type with ion-sensitive layer of silicon nitride and exchangeable alginate membranes with immobilized yeast cells Candida krusei UKM Y-823, Saccharomyces cerevisiae UKM Y-517 and Cryptococcus laurentii UKM Y-1135 for simultaneous determination of glucose and sucrose concentration has been developed. The multibiosensor operation is based on the selectivity of used yeast cells towards carbohydrates and proportional generation of acid when the carbohydrates are used by yeast cells as a substrate. The measurable glucose and sucrose concentration ranges are 2-30 mM and 2-50 mM, correspondingly. The accuracy of glucose and sucrose detection in these ranges is about 5-10 %.

Key words:  multibiosensor, yeast, glucose, sucrose.